The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions. Mixing together the three Micro BCA reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Stability-kit is stable at room temperature prepared working reagent stable for up to 24 hours.Compatibility-unaffected by typical concentrations of most ionic and non-ionic detergents.Colorimetric-measure with a standard spectrophotometer or plate reader at 562 nm.Working range-linear working range for BSA of 0.5 to 20 µg/mL.to get values for any given X with eference trendline equation excel questions. Sensitivity-accurately detect down to 0.5 µg/mL (2 µg/mL in microplate format) 2017 This is the formula to obtain 2nd Order Polynomial Trendline: y.The Pierce Micro BCA Protein Assay Kit is a three-component version of our BCA reagents, optimized to measure total protein concentration of dilute protein solutions (0.5 to 20 µg/mL).Ĭompare all available BCA protein assays ›įeatures of the Micro BCA Protein Assay Kit include:
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